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Description

It catalyzes the reductive transfer of an amino group from aspartate to -ketoglutarate to yield oxaloacetate and glutamate

Metformin is a commonly used drug for the clinical treatment of T2DM, which improves the sensitivity and utilization of insulin in body tissues and reduces the occurrence of adverse events such as hypoglycemia and obesity (Pernicova and Korbonits, 2014)

To investigate the function of CBS in this process, we silenced its expression by CRISPR/cas9 mediated gene knockout strategy (sg CBS ) in SCD erythroblasts (Fig