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It is an essential nutrient, meaning that it cannot be synthesized by the body and must be obtained through diet or supplements

Precipitated proteins were spun down and resuspended in GdmCl lysis buffer (6 M guanidine hydrochloride, 5 mM tris(2-carboxyethyl)phosphine, 10 mM chloroacetamide, 100 mM Tris pH 8.5, 1 complete mini protease inhibitor cocktail tablet per 10 ml, 5 mM sodium fluoride, 1 mM sodium orthovanadate, 5 mM -glycerol phosphate)

Adding more water changes concentration, not the amount of peptide present