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Quantification of the target analytes (MK4 and MK4 epoxide) was achieved using an Agilent 5890 Series II gas chromatograph (GC) coupled with a Thermo Finnigan SSQ7000 single quadrupole mass spectrometer (MS)

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As the medium already contains 200 M methionine and 400 M serine, the relative proportion of the labelled species is 50 and 33%, respectively