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This gets controversial as I know some say it is just the doctors doling out medication or teachers expecting too much too soon, but as a collective whole, children really are having more attention problems than in the past

The protein blots were incubated with first antibody for 2 h at room temperature, then probed with IRDye infrared secondary mouse or rabbit anti-rabbit IgG and visualized using the odyssey infrared imaging system (LI-COR Biosciences, Lincoln, NE, USA)