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However, whereas HIF-1 is expressed in all nucleated cells in most of the metazoan species, HIF-2 and HIF-3 are only expressed in certain cell types in vertebrate species [19]

A pRS315- HS-pGSH1-URA3-GFP-tADH1-HS plasmid was subsequently constructed using these and GFP (above) fragments, with the same Gibson approach as described earlier