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[0249] Metabolic Stability in SIF and SGF (Simulated Intestinal and Gastric Fluid) Assay Conditions [Drug] = 5 Buffer 1 = SGF without enzyme Buffer 2 = SGF with 0.32% pepsin Buffer 3 = SIF without enzyme Buffer 4 = SIF with 1% pancreatin Time = 0, 60, 120, and 240 min Temperature = 37C Experimental Protocol SIF was prepared freshly with 8.7 mM NaOH, 28.65 mM NaH2P04, 105.85 mM NaCl, with a final pH of 6.8

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NA, Noradrenaline

It holds the potential to elucidate the impact of cellular growth conditions such as heat-shock, starvation, or UV damage on the 20S substratome, while also uncovering differences between diverse cell types