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Bristow DJ, Holmes DS: Cortisol levels and anxiety related behaviours in cattle

Buccellato, F

doi: 10.1038/s41598-024-59677-1 141 WangBShangguanWLiWXieMYuYYangQet al

This megaprimer is used to prime a PCR reaction (PCRClone) with the linearized pCS2+8 and the crude mixture (with or without Dpn1 digestion) is directly transformed into E