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2023 September 1511 (5):e0228523

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doi: 10.1007/s11064-022-03726-6 58

For Atto594 labeling, Atto594-OSu ester (AttoTec) was solved in DMSO, and 2 l of this stock solution (7 mM) was incubated with 2 l of amino-modified ASO (6 g l 1 ) in 16 l of labeling buffer (20 parts PBS, pH 7.4, plus 1 part 0.2 M aqueous sodium bicarbonate, pH 9) at room temperature overnight