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Developing Enhanced BloodBrain Barrier Permeability Models: Integrating External Bio-Assay Data in QSAR Modeling

The total gradient time was 19 min and went from 8% to 24% acetonitrile (ACN) in 12.5 min, followed by 2.5 min to 36%

However, this strategy is only attractive if the therapeutic agent itself is not a substrate for CPA, or if the case, that its functional activity is not negatively affected by C-terminal cleavage