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Cytokine production assays T cells and tumor cells (E:T as specified in figure legends) were cocultured in 250 l media without IL-2 in round bottom 96-well plates for 24 h

There are several nutrients of note necessary to produce glutathione and for which studies have found that increasing the amount relates to a boost in glutathione levels

That distinction matters for understanding why systemic IGF-1 LR3 administration and local MGF activity are not equivalent in their biological targets, even though both ultimately activate many of the same downstream signalling nodes