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To determine the effect of ferroptosis, HepG2 and SNU398 cells with or without SLC13A3 knockdown were treated with sorafenib (0, 1.25, 2.5, 5, and 10 M), erastin (0, 1.25, 2.5, 5, and 10 M), RSL3 (0, 0.025,0.05, 0.1, and 0.5 M), BSO (0, 1.25,2.5, 5, and 10 mM) or AF (0, 1.25, 2.5, 5, and 10 M) for 48 h