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ChIP-seq For ChIP-seq assays, parental and stably expressing GFP, GFP-ASXL1 1-645 -WT or GFP-ASXL1 1-645 -I574D HEK293T cells (ATCC) were maintained in Dulbeccos Modified Eagle Medium (Gibco), supplemented with 10% FBS (Sigma), 1% penicillin-streptomycin, and 2 mg/ml puromycin

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TJ paracellular barrier function is based on two independently regulated routes 37 , namely the pore pathway: a highly selective route for the flux of ions and water, that is regulated by claudins

They are NOT a troche and are taken by placing it in the back of the mouth and drinking it down with a liquid so as not to have a blue mouth