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Laughing gas can inactivate vitamin B12 in the body Nitrous oxide can paralyze people because it destroys nerve cells within the spinal cord by inactivating vitamin B12, which is essential for maintaining the lining of the nerves, Alistair Noyce, a consultant neurologist working in a London hospital in the UK, told Insider

More research needs to be done to confirm these benefits in humans

Read that carefully, because its a more sophisticated point than tolerance. Its not that receptors get tired

Similarly, activating the GABA-A receptor on other immune system cells, such as mast cells, natural killer cells, macrophages, or dendritic cells, also inhibits their inflammatory activity.[ref][ref][ref][ref] A question that puzzled researchers for a while was the source of GABA in the immune system