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Using highly multiplexed antibody staining with imaging mass cytometry, we identified the emergence of stress-associated microglia (SAM) using a spatial proteomics method in the brains of 2HIT mice, which highly express TGF, APOE, MHC-class II, IL-6, and TREM2 proteins

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Finally, to confirm that the M105I mutation induces modifications in the -SNAP protein that alters its lipid binding affinity, and to rule out the possibility that the reduction in PM binding is due to differential interactions with membrane-associated proteins, we performed a liposome flotation assay