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Briefly, tissue sections were incubated with primary antibodies at a suitable concentration at 4 C overnight

Concepts such as virtual digital twins for nutrition envision a computational replica of an individual that integrates genetic, metabolomic, immune, microbiome and behavioral data to simulate the impact of dietary interventions in silico (254)

However, this study did not investigate other environmental or dietary factors that might influence DNA methylation, such as alcohol consumption [40]

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