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Finally, induction of the expression and activity of GSTs and other phase II detoxification/antioxidant enzymes by isothiocyanates is an important defense mechanism against oxidative stress and damage associated with the development of diseases like cancer and cardiovascular disease (22)

The regulation of the Nrf2/ARE pathway after VACV infection was investigated using a luciferase assay, which demonstrated a significant increase in the ratio between ARE-dependent Firefly luciferase activity and ARE-independent Renilla luciferase activity (Fig