glutathione adduct formation toxicity Frontiers Glutathione-Mediated Conjugation of Anticancer Drugs:

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RNA extraction, cDNA synthesis, quantitative RTPCR After tissue homogenizing, the total RNA from EFT-412 tissues was extracted with Trizol (Life Sciences) and purified using the RNeasy Mini Kit (Qiagen) according to the manufacturers instructions

The generation of ROS by embryo metabolism or the culture medium has been demonstrated to induce a series of deleterious effects, including protein denaturation, apoptosis, autophagy, lipid oxidation, DNA damage, and mitochondrial impairment

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