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Once the needle is in place, gently pull back on the plunger just a tiny bit

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Consistent with the results in male mice, Sry -TG female mice were also more susceptible to APAP-induced liver injury, as indicated by higher levels of ALT and AST (Figure S7C) and a greater proportion of necrotic tissue than observed in APAP-treated WT female mice (Figure S7 A, D)