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Scores on the Three-Factor Eating Questionnaire (TFEQ-R18) improved in all treatment groups, with Cagrilintide producing greater improvements than placebo in specific domains: cognitive restraint, notably at 1.2 mg and 4.5 mg, emotional eating notably at 0.3 mg and 2.4 mg, and uncontrolled eating notably at 1.24.5 mg

Proteins , 2016, vol

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Quantitative real-time polymerase chain reaction (qRT-PCR) assays Total RNA was extracted from cells using the TRIzol reagent (YY101, Epizyme), and cDNA was synthesized by reverse transcription using the 5 All-In-One RT MasterMix (G490, ABM) according to the manufacturers protocol