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As a result, we also finalized our proposal that the ASC payment rates for these ( services would not be reduced for failure to meet the ASCQR Program requirements because the payment rates for these services are not calculated using the ASC conversion factor and, therefore, are not affected by reductions to the annual update (77 FR 68500)

Flow cytometry intracellular immunostaining Nrf2 wt/wt - and Nrf2 / -derived cells were collected at day 22 (cAMP condition) and prepared for flow cytometry immunostaining as follows: Matrigel-embedded organoids were digested using a HBSS solution containing 10 U/ml dispase II (Roche) and 125 U/ml of collagenase type IV (Gibco, Thermo Fisher Scientific) for 3060 min at 37C