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Figure 5 Regulatory Factor, Lipolytic and Lipogenic Pathway Key Markers In order to determine the mechanism of Sirt1/Srebp-1/Ppar pathway-mediated dietary betaine regulation of lipid metabolism, the expression of genes related to several lipid metabolic pathways including regulatory and transcriptional factors, lipolytic pathway and lipogenic pathway were determined in the liver of black seabream juveniles ( Figure 6 )