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The study protocol was approved by the ethics committee of Korea University Hospital, and the study was conducted in accordance with the guidelines of the Helsinki Declaration

The remaining blood samples were centrifuged at 3000 rpm for 15 min after clotting, and the separated sera were kept at 20 C until the biochemical analysis

As previously reported, PLAL at the water-air interface by simultaneous pulses at the wavelength of 1064 nm and 532 nm, leads to ultrasmall nanoparticles with an average diameter of about 3 nm, which are in part embedded in the organic acids produced during the pulsed laser driven CO 2 reduction reaction [15]

Thus, an increase in NRF2 activity, reflected by induction of NRF2 target genes, likely represent an important mechanism mediating the protection seen in GCLM KO mice