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They are recognized by PRRs [e.g., lipopolysaccharide (LPS) from Gram-negative bacteria (recognized by TLR4), flagellin (TLR5), lipoteichoic acid and peptidoglycan from Gram-positive bacteria (TLR2), viral nucleic acids, like double-stranded RNA (TLR3) and unmethylated CpG DNA (TLR9)], triggering the innate immune response

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There is still an ongoing debate on whether the three CTA models are interchangeable or whether there should be criteria guiding the choice of one over the other based on their peculiarities and the characteristics of the tested chemicals ( In the absence of approved test guidelines, the OECD issued two guidance documents endorsing the use of CTA based on SHE and Bhas 42 cells