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10.4093/dmj.2018.0090 83 LeeY.-S.LeeD.ParkG.-S.KoS.-H.ParkJ.LeeY.-K.et al (2021)

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To study gene expression, total RNA was extracted from half-colon samples using phenol-chloroform methods and purified with lithium chloride precipitation, which is particularly important to remove residual DSS polysaccharides that can interfere with downstream reactions