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After 30 min, the UVvis absorption spectra at 650 nm was recorded using a Microplate Reader (Victor Nivo , PerkinElmer, USA)

Let's say, for example, your protocol calls for a working solution where each unit of volume (e.g., 0.1 mL) contains a specific amount of 5-Amino-1MQ (e.g., 5 mg)

Finally, PPIs inhibit lysosomal acidification by suppressing the neutrophil vacuolar-type H + ATPase (V-ATPase), which ultimately reduces neutrophil antibacterial activity (Song et al., 2017)