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Supplementary Validation Method: LCMS/MS For high-sensitivity detection requirements, liquid chromatographytandem mass spectrometry is used for supplementary validation, especially suitable for trace component analysis and metabolic studies

456 In the spinal cord, the influx of neuropathic signals to the dorsal horn leads to central sensitization, characterized by increased spontaneous neuronal activity, lowered activation thresholds for peripheral stimuli, heightened responses to sub-threshold stimuli, and expansion of receptive fields

Vitamin B12 is water-soluble it quickly dissolves in water and does not remain in the body, being eliminated through urine within 2472 hours depending on physical activity and water intake

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