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Coulomb interactions were calculated using the particle-mesh Ewald (PME) algorithm

In this context, pro-inflammatory cytokines (e.g., TNF, IL-1, IL-8) can activate NET formation ( In the context of CVD, NETs not only exert cytotoxic and prothrombotic effects (Warnatsch et al., 2015), but also contribute to plaque formation (Silvestre-Roig et al., 2019)