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10.3390/molecules24213892 207 TianY.LeiY.WangY.LaiJ.WangJ.XiaF

RAF proteins are, in turn, activated by RAS, a GTP-binding protein that undergoes a GDP-to-GTP transition in response to extracellular stimuli, directly interacting with RAF to initiate the Raf/MEK/ERK cascade (71)

GoldBio Nickel Agarose EDTA and DTT Maximum Concentrations As we will discuss in the next section, using too much EDTA or DTT will strip the nickel off of your agarose beads, or render them incapable for binding to his-tags