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Then cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kits (4374967, Applied Biosystems) with the accompanied random primers, as described previously ( The mRNA expression levels of given genes in the transfected cells with or without cell sorting enrichment were determined by qPCR using Taq388 mix (Du et al., 2022) on a QIAGEN Q-Rex machine (Qiagen, Germany)

Subsequently, the cells were seeded in a 6-well plate at a density of 3 10 6 cells per well

Overview of ferroptosis and its signal transduction RCDs are essential for numerous biological processes, such as the maintenance of normal homeostasis and elimination of detrimental stimuli [70]

C-type cells are intermediate transient neuronal cells (nIPC) that proliferate rapidly and differentiate into neuroblasts or type-A cells (Nualart et al., 2012