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Hydrogen sulfide perturbs mitochondrial bioenergetics and triggers metabolic reprogramming in colon cells

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MDA-MB-231 and MDA-MB-468 cells treated with 5 M R001 for 24 h, processed and analyzed by flow cytometry for cell cycle progression showed an altered cell cycle profile, with the accumulation of cells at both G1 (58.4 vs 38.9) and in G2 (20.2 vs 10.8) phases, and decreased S-phase population (20.5 vs 49.8) for MDA-MB-231, and largely an accumulation of cells in the G2 phase (21 vs 10.8) for MDA-MB-468, comparing the treated to untreated (DMSO) control cells (Fig