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Use sterile technique, swirl gently to mix, and then recalculate your concentration using the new total water volume

3c) to directly observe the cellular targets

Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen, 0.5 raM L-glutamine, and 5mM cytosine-D-arabinofuranoside from Sigma added at 2 days in vitro