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This phenomenon may be attributed to two primary factors: first, the high quality sperm obtained through PureSperm density gradient centrifugation may play a crucial role, which has been demonstrated to significantly reduce apoptotic sperm and cellular debris while maintaining a viable sperm recovery rate exceeding 85% [47], second, the supplementation of Vit A and Lup may effectively enhance sperm motility, plasma membrane integrity, acrosome integrity, and antioxidant capacity while reducing sperm apoptosis levels

16 and MDA levels were assessed according to the method of Ohkawa et al

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Although blood-based tau biomarkers have demonstrated high diagnostic performance, additional accessible biofluids may provide alternative molecular information and further support screening and disease monitoring strategies