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the use of pro-GSH molecules (Fraternale et al., 2006) and/or glutathione precursors like N-acetyl cysteine (NAC), glutamine, cysteine (cystine) and glycine, as well as nuclear factor erythroid 2 p45related factor 2 (Nrf2) inducers like sulforaphane can enhance glutathione production and increase nuclear Nrf2 translocation and antioxidant response element (ARE) transcription (Komaravelli and Casola, 2014

Effect of thioacetamide on hepatic -glutamylcysteine synthetase subunit expression

Expressing the dominant negative mutant H3.3Q5A in the VTA significantly reduced seeking behavior after heroin withdrawal, with behavioral tests showing over a 50% reduction in active responses, highlighting its role in reuptake susceptibility

While oral and topical applications of glutathione are generally considered safer, intravenous therapies are a strict no-go