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(link: ( Project accession: PXD063438

To elute the antigen-antibody complex, beads were heated at 99 C in 1 loading buffer for 5 min

doi: 10.1053/j.semnuclmed.2022.04.004 323 GoodburnRJPhilippensMEPLefebvreTLKhalifaABruijnenTFreedmanJNet al

Connection 4 BH4 Depletion and eNOS Uncoupling Beyond Homocysteine Beyond its homocysteine-mediated BH4 oxidation effect, MTHFR impairment reduces BH4 recycling through a second mechanism: the dihydrobiopterin reductase (DHFR) enzyme that regenerates BH4 from BH2 requires dihydrofolate reductase activity an enzyme that uses NADPH to reduce both dihydrofolate to THF and BH2 to BH4