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To elucidate the role of R-loops in DNA replication stress induced by overexpression of HRASV12 or cyclin E1, we introduced the retroviruses encoding these oncogenes into U2OS T-REx cells harboring an RNASEH1 transgene under the control of a doxycycline-regulated CMV promoter (U2OS T-REx [RNH1-GFP]) 32

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