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For samples frozen on holey carbon grids, the Vitrobot was maintained at 4LC with 100% humidity, using blotting times of 3.5Ls to 4.5Ls, blotting force of 0, and a waiting time of 10Ls

Notably, elevated intestinal permeability has been implicated in IBD (Yu et al., 2022), celiac disease (Gundry, 2020) and Type 1 diabetes (Miele et al., 2020)