A half-media change was carried out every 3 days for high-density cultures used for biochemistry and every 5 days for low-density cultures used for imaging
( A ) Relative GSH and GSSH levels in HeLa and Pfa1 cells as measured by LC-MS
the BPC157 The technical solution provided by the present invention is specifically a solid-phase preparation method of BPC157 peptide, comprising the following steps: 4--4--2--FmocBPC157FmocDIC/HOBtDIC/HOAtBOP/HOBtBOP/HOAtHBTU/HOBtHBTU/HOAtHATU/HOBtHATU/HOAtHCTU/HOBtBPC157BPC157C 18 C 8 BPC157 Use trityl chloromethyl resin as synthetic resin, including 4-methyl-trityl chloromethyl resin, 4-methoxy-trityl chloromethyl resin, 2-chloro-trityl chloromethyl resin or Wang's Resin, with any one of the above resins as the starting material, according to the method of solid-phase synthesis, the amino acids with Fmoc protection are sequentially connected to obtain the protected BPC157 peptide resin, during which the Fmoc protection group is sequentially removed, and the DIC/HOBt Or one of DIC/HOAt or BOP/HOBt or BOP/HOAt or HBTU/HOBt or HBTU/HOAt or HATU/HOBt or HATU/HOAt or HCTU/HOBt is a condensing agent for peptide grafting reaction, and the protected BPC157 peptide resin Afterwards, remove the side chain protection group and cut the peptide simultaneously to obtain the crude product of BPC157 peptide, which is separated and purified by C 18 or C 8 chromatographic column to obtain the desired pure product of BPC157 peptide
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