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Exposing neurons to pathological tau-seeding materials, typically derived by isolating insoluble tau fractions from human diseased brains, is one common method used to induce tau accumulation in mice 2,3,4,5,6,7,8,9,10,11 and primary neurons 4,5,10,12

Nat Rev Immunol (2020) 20(6):35562

The second common path to OTC approval is under the new drug application process

On the other hand, if the drug accidentally enters the left ventricular cavity, all body tissues may be exposed to the drug, reducing its therapeutic efficacy and leading to unexpected outcomes [185, 232, 233]