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A paradigm for assessing glycoside binding affinities and selectivities by 1H NMR spectroscopy

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Drug-specific variables that may affect half-life Drug formulation (ie, modified or controlled release preparations extend half-life) How the drug behaves in the body (ie, zero-order, first-order, or multi-compartmental pharmacokinetics) How the drug is administered (half-life may be different with IV administration, compared to intranasal or oral administration) How the drug is cleared from the body (eg, kidneys, liver, lungs) If the drug accumulates in fat or other types of tissue If the drug binds to proteins or not Presence of metabolites or other drugs that may interact Properties of the drug, including molecule size, charge, and pKa The volume of distribution of a drug Other variables, such as if the drug is actively transported, is self-induced, or has saturation pharmacokinetics