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These findings underscore lipid peroxide accumulation as a hallmark of ferroptosis, and suggest that downregulating ACSL4, LPCAT3, LOXs, or POR can suppress PUFA-PL-OOH formation and effectively inhibit ferroptosis

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The tradeoff is convenience measuring powder is less portable than taking a capsule, and some people may find NAC's sulfur-like taste unpleasant

The lysis buffer was prepared by mixing 10 ml of M-PER (ThermoFisher) with 100 l of Protease & Phosphatase inhibitor mix (ThermoScientific) and 100 l of 0.5 M EDTA (ThermoScientific)