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Started with 0.25 mg once daily, injecting subcutaneously about two inches from where the pain was worst

The animals were placed in an induction chamber with an oxygen-enriched environment, with the initial induction concentration set at 4%5% isoflurane, until deep anesthesia signs such as loss of the righting reflex appeared, after which maintenance was initiated

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This question determines how many 100mg vials you'll need for a complete protocol, and whether your single vial will suffice