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These metabolites, in turn, can feedback into the brain through the bloodstream, immune system signaling, or vagal nerve pathways, thereby influencing central immune responses and the homeostasis of the BBB (173)

In fact, we estimated that peptides capable of interfering with GAL4-DBD activity would be less represented with respect to those which are simply bait-interacting peptides (i.e., interaction without negative effect on GAL4-DBD), as their selection is unfavored by the choice to target an essential yeast two-hybrid module

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