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[129] Telephone interview with Mona Hanna-Attisha, October 2016

cDNA was synthesized using i-script cDNA synthesis kit (Bio-Rad) as per the manufacturers instructions

Reported approaches include LDM (Wellcomet, Germany) ultrasound for the mechanical dispersion of tissue and trapped blood components, PICO laser for pigment fragmentation, radiofrequency (RF) to soften the scaffoldtissue complex, long-pulsed Nd:YAG for targeting residual pigment molecules, IPL for vascular and pigment clearance, and an enzymatic strategy