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NMR experiments NMR spectra, both in-cell and in vitro, were collected at 308 K with a 950-MHz Bruker Avance III spectrometer, equipped with a CP TCI CryoProbe

However, SLNs have several limitations concerning their application, including unpredictable drug-release behaviour and the potential for gelation owing to the polymorphism of solid lipids [134, 135]

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Data-dependent MS/MS (MS2) analysis was conducted in top speed mode with a 1-second cycle time, where the most abundant multiple-charged ( 2+ - 5+ ) precursor ions were selected for activation in order of abundance and detected in linear ion trap at rapid scan rate