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Quantitative RT-PCR (qRT-PCR) qRT-PCR was performed according to the manufacturers instructions

We demonstrate that: (1) SC-specific GSDMD deletion protects against NIHL, (2) noise-induced oxidative stress in HCs triggers a signaling cascade via epidermal growth factor receptor/extracellular-regulated kinases (EGFR/ERK), which activates caspase-11-dependent GSDMD cleavage in SCs

Despite clear microbial shifts correlating with disease severity, these patients had lower plasma TMAO levels than asymptomatic controls, suggesting a complex interplay between gut microbiota composition and metabolic risk pathways