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After the final wash, the pellet was resuspended in 40 l of 1x electrophoresis sample buffer (40% glycerol, 240 mM Tris/HCl pH 6.8, 8% SDS, 0.04% bromophenol blue, 5% -mercaptoethanol) and analyzed by SDS-PAGE western blot

Under the influence of IL-4 and IL-13 and the interactions between adhesion molecules on B cells (class II MHC molecules: CD80/CD86, CD40, and ICAM-1) and T cells (CD3, CD4, CD28, CD40L, and LFA-1 receptors), B cells switch from expressing IgM, IgG2, and IgG3 to IgG4 and IgE (179)

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For example, Elsa et al .[6] obtained yeast extract by mechanical crushing and found the protein content to be as high as 64.1% (dw), the fat content at only 1.32% (dw), and the RNA content at 4%