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To prepare hippocampal slices, horizontal sections (400 m thick) at the middle septotemporal levels were cut in ice-cold cutting solution using a Leica VT1000S vibratome (Leica Microsystems) and allowed to recover at 32 C for at least 1 h in an oxygenated (95% O 2 /5% CO 2 ) artificial cerebrospinal fluid (aCSF) solution (124 mM NaCl, 2 mM KCl, 2 mM MgSO 4 , 2 mM CaCl 2 , 1.25 mM NaH 2 PO 4 , 26 mM NaHCO 3 , and 10 mM Glucose, with pH 7.3 and osmolarity 300 mOsm/L)

(1077) Franco-de-Moraes, A

indicated by P-values (h-j)

This protective response might be an attempt to reduce the levels of oxidative stress but alternatively might accelerate ferroptosis in case of excessive HMOX-1 activation [30]