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Or non-biospecific, such as: proteins binding to the dye substance, histidine-proteins binding to metal ions (immobilized metal ion affinity chromatography- IMAC)

10.1021/ja00969a017 72 LiuJ.SunZ.YuanY.TianX.LiuX.DuanG.et al (2016)

To provide a more accurate prediction of skin irritancy potential of the test compound, in vitro skin irritation test can include other potential biomarkers