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Cells were incubated with 1 M SIINFEKL (OVA peptide), SVLAFRRL (Tgd057 peptide) or AVEIHRPVPGTAPPS (AS15 peptide) and fluorescently labelled LAMP1 antibody for 2 h, followed by a further 2 h with Protein Transport Inhibitor Cocktail (Invitrogen: 00-4980-03)