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Results Characterization of repRNA encoded gH/gL and gH/gL/gp42 To enable co-delivery of both gH and gL or gH, gL, and gp42 on a single repRNA, we designed two constructs

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RBCs that aggregated as the bottom layer were collected and stored at -20C in a cell lysis/protein storage buffer [20 mM Tris, 100 mM NaCl (Amresco, N653), 1X protease inhibitor cocktail (Amresco, M221)] for western blot analysis